Bias and artifacts in multitemplate polymerase chain reactions (PCR)

doi:10.1016/S1389-1723(03)90130-7

Journal of Bioscience and Bioengineering

Volume 96, Issue 4, 2003, Pages 317-323

https://doi.org/10.1016/S1389-1723(03)90130-7 Get rights and content

Abstract

Polymerase chain reaction (PCR) is often used for the amplification of a mixture of homologous genes. PCR bias and artifact formation can occur in multitemplate PCR, and provide incorrect information on the abundance and diversity of genes. PCR bias and artifact formation occur at a higher rate during the last few cycles of the reaction, and therefore can be avoided by stopping the PCR earlier.

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ReviewBias and artifacts in multitemplate polymerase chain reactions (PCR)

Abstract

J. Biol. Chem.

J. Biosci. Bioeng.

J. Biosci. Bioeng.

J. Biosci. Bioeng.

Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR

Appl. Environ. Microbiol.

DNA rehybridization during PCR: the ‘C0t effect’ and its consequences

Nucleic Acids Res.

Bias in template-toproduct ratios in multitemplate PCR

Appl. Environ. Microbiol.

Quantitative measure of small-subunit rRNA gene sequences of the kingdomKorarchaeota

Appl. Environ. Microbiol.

DNA recombination during PCR

Nucleic Acids Res.

Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed-culture of strict barophilic bacteria

Microb. Ecol.

Template-switching during DNA synthesis byThermus aquaticus DNA polymerase I

Nucleic Acids Res.

Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide

The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species

Microbiology

Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes

Appl. Environ. Microbiol.

Stimulation and suppression of PCR-mediated recombination

Nucleic Acids Res.

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences

Appl. Environ. Microbiol.

Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning

Appl. Environ. Microbiol.

Fluorescence-based method for measuring and determining the mechanisms of recombination in quantitative PCR

Clin. Chim. Acta

Review
Bias and artifacts in multitemplate polymerase chain reactions (PCR)

DNA rehybridization during PCR: the ‘C₀t effect’ and its consequences