Notes & TipsAn acetyltransferase assay for CREB-binding protein based on reverse phase–ultra-fast liquid chromatography of fluorescent histone H3 peptides
Section snippets
Acknowledgments
This work was supported by the Institut National du Cancer (INCa, 2012-1-PL-BIO 2012, coordinated by S. Ait-Si-Ali) and the ITMO Cancer (plan Cancer-Environnement 2013, coordinated by C. Chomienne and F. Rodrigues-Lima). R.D. and C.M. are supported by PhD fellowships from the Région Ile-de-France and Université Paris Diderot, respectively. UFLC analyses were done on the platform “Bioprofiler” (Unité de Biologie Fonctionnelle et Adaptative, BFA).
References (16)
- et al.
Assays for mechanistic investigations of protein/histone acetyltransferases
Methods
(2005) - et al.
Fluorescent reporters of the histone acetyltransferase
Anal. Biochem.
(2008) - et al.
A high-performance liquid chromatography assay for Dyrk1a, a Down syndrome-associated kinase
Anal. Biochem.
(2014) - et al.
Application of a fluorescent histone acetyltransferase assay to probe the substrate specificity of the human p300/CBP-associated factor
Anal. Biochem.
(2000) - et al.
Small molecule modulators of histone acetyltransferase p300
J. Biol. Chem.
(2003) - et al.
50 years of protein acetylation: from gene regulation to epigenetics, metabolism and beyond
Nat. Rev. Mol. Cell Biol.
(2015) - et al.
Transcriptional/epigenetic regulator CBP/p300 in tumorigenesis: structural and functional versatility in target recognition
Cell. Mol. Life Sci.
(2013) - et al.
Differences in specificity and selectivity between CBP and p300 acetylation of histone H3 and H3/H4
Biochemistry
(2013)
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