Archival ReportStress-Induced Redistribution of Corticotropin-Releasing Factor Receptor Subtypes in the Dorsal Raphe Nucleus
Section snippets
Subjects
Adult male Sprague-Dawley rats weighing 250–300 g were housed 2–3 per cage on a 12-hour light schedule in a temperature-controlled (20°C) colony room with free access to standard chow and water. Rats used in fluorescent microscopy studies were obtained from Harlan Laboratories (Indianapolis, Indiana), and rats used in swim stress studies were from Taconic Laboratories (Germantown, New York). All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Thomas
CRF Receptor Localization in DR Neurons of Unstressed Rats: Light Microscopy
CRF1 immunoreactivity was robust in the perikarya of DR neurons, where it was associated with both 5-HT- and non-5-HT-containing cell bodies (Figure 1A,1C, and 1E). Within these profiles, CRF1 immunoreactivity was most prominent near the periphery, consistent with a localization on the plasma membrane (Figure 1A, 1C, and 1E).
The appearance of CRF2 immunolabeling in the DR differed from that of CRF1 in being more punctate (Figure 1B, 1F, and 1H). Like CRF1, CRF2 was associated with 5-HT- and
Discussion
These findings are the first to describe the cellular localization of CRF receptor subtypes CRF1 and CRF2 in the DR at an ultrastructural level and demonstrate that these are differentially distributed in cellular compartments, with CRF1 having an increased presence on the plasma membrane compared with CRF2 in unstressed animals. Swim stress results in trafficking of the CRF receptor subtypes in opposing directions such that CRF1 tends to move to the cytoplasm and CRF2 is recruited to the
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