Biophysical Journal
Volume 98, Issue 7, 7 April 2010, Pages 1160-1169
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Article
Stationary Gating of GluN1/GluN2B Receptors in Intact Membrane Patches

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Abstract

NMDA receptors are heteromeric glutamate-gated channels composed of GluN1 and GluN2 subunits. Receptor isoforms that differ in their GluN2-subunit type (A–D) are expressed differentially throughout the central nervous system and have distinct kinetic properties in recombinant systems. How specific receptor isoforms contribute to the functions generally attributed to NMDA receptors remains unknown, due in part to the incomplete functional characterization of individual receptor types and unclear molecular composition of native receptors. We examined the stationary gating kinetics of individual rat recombinant GluN1/GluN2B receptors in cell-attached patches of transiently transfected HEK293 cells and used kinetic analyses and modeling to describe the full range of this receptor's gating behaviors. We found that, like GluN1/GluN2A receptors, GluN1/GluN2B receptors have three gating modes that are distinguishable by their mean open durations. However, for GluN1/GluN2B receptors, the modes also differed markedly in their mean closed durations and thus generated a broader range of open probabilities. We also found that regardless of gating mode, glutamate dissociation occurred ∼4-fold more slowly (k = 15 s−1) compared to that observed in GluN1/GluN2A receptors. On the basis of these results, we suggest that slow glutamate dissociation and modal gating underlie the long heterogeneous activations of GluN1/GluN2B receptors.

Abbreviations used

NMDA
N-methyl-d-aspartate
Glu
glutamate
Gly
glycine
MOT
mean open time
MCT
mean closed time
Po
open probability
HEPBS
N-(2-hydroxyethyl)piperazine-N′-(4-butanesulfonic acid)
GluN1
glycine-binding NMDA receptor subunit (formerly NR1)
GluN2
glutamate-binding NMDA receptor subunit (formerly NR2)

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