Elsevier

Neuroscience Letters

Volume 454, Issue 2, 24 April 2009, Pages 129-133
Neuroscience Letters

Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells

https://doi.org/10.1016/j.neulet.2009.02.069Get rights and content

Abstract

TREK belongs to a subfamily of tandem pore domain K+ channels, and consists of three subunits, TREK-1, TREK-2 and TRAAK. We examined the distribution of TREK-1, TREK-2 and TRAAK immunoreactive neurons in rat trigeminal sensory neurons. In the trigeminal ganglia, 31%, 43% and 60% of neurons were immunoreactive for TREK-1, TREK-2 and TRAAK, respectively. Mean sizes of TREK-1, TREK-2 and TRAAK immunoreactive trigeminal ganglion neurons were 447 ± 185, 445 ± 23 and 492 ± 12 mm2, respectively. Furthermore, TREK channels were colocalized with cationic TRP channels, TRPV1, TRPV2 and TRPM8. TREK-1 immunoreactive neurons were colocalized with TRPV1 (57%), TRPV2 (11%) and TRPM8 (33%). TREK-2-immunoreactive neurons were colocalized with TRPV1 (33%), TRPV2 (9%) and TRPM8 (19%). TRAAK immunoreactive neurons were colocalized with TRPV1 (47%), TRPV2 (10%) and TRPM8 (22%). The present results revealed that TREK-1, TREK-2 and TRAAK channels colocalized with thermosensitive TRP channels in some small trigeminal ganglion neurons.

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Acknowledgements

This study was supported in part by a Grant-in-Aid from the JSPS, Japan (19380166).

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    Surprisingly, this low level of identity does not preclude heteromerization, as we and others recently showed within the TREK subfamily (Blin et al., 2016; Hwang et al., 2014; Lengyel et al., 2016; Levitz et al., 2016). Based on this and the fact that TG neurons express many K2P channels (TREK1, TREK2, TRAAK, TASK1, and TASK3; Bautista et al., 2008; Yamamoto et al., 2009), we hypothesized that the difference between TRESK mutants is due to their differential ability to modify the function of other K2P channels through heteromerization. To assess the ability of TRESK to heteromerize with other K2P channels that are expressed in TG neurons, we used the single-molecule pull-down (“SiMPull”) assay (Jain et al., 2012) to visualize individual antibody-immobilized protein complexes on polyethylene-glycol-passivated glass coverslips (Figure 1A).

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