Cell Stem Cell
Volume 9, Issue 1, 8 July 2011, Pages 74-86
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Article
An Isoform of Decorin Is a Resistin Receptor on the Surface of Adipose Progenitor Cells

https://doi.org/10.1016/j.stem.2011.05.017Get rights and content
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Summary

Adipose stromal cells (ASCs) serve as mesenchymal progenitors in white adipose tissue (WAT). Intercellular interactions involving ASCs have remained obscure. By merging phage display technology with fluorescence-activated cell sorting (FACS), we screened a combinatorial library for peptides that target mouse ASCs in vivo. We isolated peptide CSWKYWFGEC that specifically homes to ASCs, used it as bait to purify the corresponding ASC surface receptor, and identified it as a previously unreported cleavage product of decorin (DCN) lacking the glycanation site (termed ΔDCN). We demonstrate that ΔDCN is differentially expressed on ASC surface. In a screen for ΔDCN-binding proteins, we identified resistin, an adipokine for which the receptor has been unknown. Expression of ΔDCN in 3T3-L1 cells promoted proliferation and migration but suppressed lipid accumulation upon adipogenesis induction, which was resistin dependent. We conclude that ΔDCN serves as a functional receptor of resistin in adipocyte progenitors and may regulate WAT expansion.

Highlights

► Phage display identifies peptides that home to adipose stromal cells (ASCs) in vivo ► A nonglycanated isoform of decorin (ΔDCN) is isolated as a peptide target ► The adipokine resistin is identified as a ligand for ΔDCN on the ASC surface ► Resistin-ΔDCN interaction controls ASC proliferation, migration, and differentiation

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