Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A family of transcriptional adaptor proteins targeted by the E1A oncoprotein

Nature volume 374, pages 81–84 (1995)Cite this article

Abstract

THE cellular protein p300 is a target of the adenoviral El A oncoprotein and is thought to participate in preventing the GO/G1 transition in the cell cycle, activating certain enhancers and stimulating differentiation pathways1. CBP is a protein that is associated with and coactivates the transcription factor CREB, mediating the induction by cyclic AMP of certain responsive promoters2–4. The sequences of p300 and CBP are highly related4,5. We show here that p300, like CBP2, can stimulate transcription. This activity is directly and specifically inhibited by E1A. We also find that CBP exists in a DNA-bound complex containing a member of the CREB family and that El A and CBP interact with one another in vivo. In keeping with the idea that El A functionally targets CBP, cAMP-dependent transcription is repressed by El A. Thus, p300 and CBP define a family of transcriptional adaptor proteins that are specifically targeted by the El A oncoprotein.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Moran, E. Curr. Opin. genet. Dev. 3, 63–70 (1993).

    Article  CAS  Google Scholar 

  2. Chrivia, J. C. et al. Nature 365, 855–859 (1993).

    Article  ADS  CAS  Google Scholar 

  3. Kwok, R. P. S. et al. Nature 370, 223–226 (1994).

    Article  ADS  CAS  Google Scholar 

  4. Arias, J. et al. Nature 370, 226–229 (1994).

    Article  ADS  CAS  Google Scholar 

  5. Arany, Z., Sellers, W. R., Livingston, D. M. & Eckner, R. Cell 77, 799–800 (1994).

    Article  CAS  Google Scholar 

  6. Eckner, R. et al. Genes Dev. 8, 869–884 (1994).

    Article  CAS  Google Scholar 

  7. Berger, S. L. et al. Cell 70, 251–265 (1992).

    Article  CAS  Google Scholar 

  8. Barbeau, D., Charbonneau, R., Whalen, S. G., Bayley, S. T. & Branton, P. E. Oncogene 9, 359–373 (1994).

    CAS  PubMed  Google Scholar 

  9. Whyte, P., Williamson, N. M. & Harlow, E. Cell 56, 67–75 (1989).

    Article  CAS  Google Scholar 

  10. Janaswami, P. M., Kalvakolanu, D. V. R., Zhang, Y. & Sen, G. C. J. biol. Chem. 267, 24886–24891 (1992).

    CAS  PubMed  Google Scholar 

  11. Kalvakolanu, D. V. R., Liu, J., Hanson, R. W., Harter, M. L. & Sen, G. C. J. biol. Chem. 267, 2530–2536 (1992).

    CAS  PubMed  Google Scholar 

  12. Vairo, G., Argyriou, S., Bordun, A.-M., Whitty, G. & Hamilton, J. A. J. biol. Chem. 265, 2692–2701 (1990).

    CAS  PubMed  Google Scholar 

  13. Kato, J., Matsuoka, M., Polyak, K., Massagué, J. & Sherr, C. J. Cell 79, 487–496 (1994).

    Article  CAS  Google Scholar 

  14. Jones, K. W., Shapiro, M. H., Chevrette, M. & Fournier, R. E. K. Cell 66, 861–872 (1991).

    Article  CAS  Google Scholar 

  15. Boshart, M., Weih, F., Nichols, M. & Schütz, G. Cell 66, 849–859 (1991).

    Article  CAS  Google Scholar 

  16. Burgering, B. M. T., Pronk, G. J., van Weeren, P. C., Chardin, P. & Bos, J. L. EMBO J. 12, 4211–4220 (1993).

    Article  CAS  Google Scholar 

  17. Cook, S. J. & McCormick, F. Science 262, 1069–1072 (1993).

    Article  ADS  CAS  Google Scholar 

  18. Muchardt, C. & Yaniv, M. EMBO J. 12, 4279–4290 (1993).

    Article  CAS  Google Scholar 

  19. Li, R., Knight, J. D., Jackson, S. P., Tjian, R. & Botchan, M. R. Cell 65, 493–505 (1991).

    Article  CAS  Google Scholar 

  20. Stein, R., Corrigan, M., Yaciuk, P., Whelan, J. & Moran, E. J. Virol. 64, 4421–4427 (1990).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Ausubel, F. M. et al. Current Protocols in Molecular Biology (Greene and Wiley-lnterscience, New York, 1989).

    Google Scholar 

  22. Kaelin, W. G. Jr et al. Cell 70, 351–364 (1992).

    Article  CAS  Google Scholar 

  23. Shirodkar, S. et al. Cell 68, 157–166 (1992).

    Article  CAS  Google Scholar 

  24. Barberis, A., Superti-Furga, G. & Busslinger, M. Cell 50, 347–359 (1987).

    Article  CAS  Google Scholar 

  25. Montminy, M. R., Sevarino, K. A., Wagner, J. A., Mandel, G. & Goodman, R. H. Proc. natn. Acad. Sci. U.S.A. 83, 6682–6686 (1986).

    Article  ADS  CAS  Google Scholar 

  26. Mellon, P. L., Clegg, C. H., Correll, L. A. & McKnight, G. S. Proc. natn. Acad. Sci. U.S.A. 86, 4887–4891 (1989).

    Article  ADS  CAS  Google Scholar 

  27. Flemington, E. K., Speck, S. H. & Kaelin, W. G. Jr Proc. natn. Acad. Sci. U.S.A. 90, 6914–6918 (1993).

    Article  ADS  CAS  Google Scholar 

  28. Lee, C. Q., Yn, Y., Hoeffler, J. P. & Habener, J. F. EMBO J. 9, 4455–4465 (1990).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, Massachusetts, 02115, USA

    Zoltan Arany, David Newsome, Elizabeth Oldread, David M. Livingston & Richard Eckner

Authors
  1. Zoltan Arany
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Newsome
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elizabeth Oldread
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David M. Livingston
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Richard Eckner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arany, Z., Newsome, D., Oldread, E. et al. A family of transcriptional adaptor proteins targeted by the E1A oncoprotein. Nature 374, 81–84 (1995). https://doi.org/10.1038/374081a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/374081a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing