Abstract
CYTOTOXIC CD4+ Thl-cells induce cell death by triggering a Fas-dependent apoptotic pathway1–6. Potential targets include activated B cells3,7, but it is not known whether the mode of B-cell stimulation influences susceptibility to Thl-mediated cytotoxicity. Here we report that CD40-Iigand-stimuIated B cells were extremely sensitive, whereas anti-IgM-stimulated B cells were resistant, to Fas-mediated apoptosis. B cells stimulated by both CD40L and anti-IgM were not susceptible to cytolysis, demonstrating that anti-IgM-mediated protection is an active, dominant process. Resistance to Thl-mediated cytotoxicity was similarly observed in CD40L-stimulated 3–83 (anti-H–2Kk,b)8 transgenic B cells co-cultured with H–2Kk or H–2Kb (but not H–2Kd) spleno-cytes. These results indicate that B cells can participate in regulating their own destruction. Protection against Fas-dependent apoptosis afforded by immunoglobulin–receptor engagement may constitute a fail-safe mechanism that eliminates bystander B cells activated by CD40L-expressing T cells, but ensures survival of antigen-specific B cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Rouvier, E., Luciani, M.-F. & Golstein, P. J. exp. Med. 177, 195–200 (1993).
Stalder, T., Hahn, S. & Erb, P. J. Immun. 152, 1127–1133 (1994).
Ju, S. T., Cui, H., Panka, D. J., Ettinger, R. & Marshak-Rothstein, A. Proc. natn. Acad. Sci. U.S.A. 91, 4185–4189 (1994).
Hanabuchi, S. et al. Proc. natn. Acad. Sci. U.S.A. 91, 4930–4934 (1994).
Vignaux, F. & Golstein, P. Eur. J. Immun. 24, 923–927 (1994).
Henkart, P. A. Immunity 1, 343–346 (1994).
Daniel, P. T. & Krammer, P. H. J. Immun. 152, 5624–5632 (1994).
Russell, D. M. et al. Nature 354, 308–311 (1991).
Ju, S.-T., Ruddle, N. H., Strack, P., Dorf, M. E. & DeDruyff, R. H. J. Immun. 144, 23–31 (1990).
Takahashi, T. et al. Cell 76, 969–976 (1994).
Lynch, D. H. et al. Immunity 1, 131–138 (1994).
Watanabe-Fukunaga, R. et al. Nature, 356, 314–317 (1992).
Lane, P. et al. J. exp. Med. 177, 1209–1213 (1993).
Heath, A. W. et al. Cell Immun. 152, 468–480 (1993).
Francis, D. A., Karras, J. G., Ke, X.-Y., Sen, R. & Rothstein, T. L. Int. Immun. 7, 151–161 (1995).
Taylor, R. B. et al. Nature New Biol. 233, 225–228 (1971).
Ogasawara, J. et al. Nature 364, 806–809 (1993).
Trenn, G., Takayama, H. & Sitkovsky, M. V. Nature 330, 72–74 (1987).
Balk, S. P., Walker, J. & Mescher, M. F. J. Immun. 126, 2177–2183 (1981).
Erb, P., Grogg, D., Troxler, M., Kennedy, M. & Fluri, M. J. Immun. 144, 790–795 (1990).
Owen-Schaub, L. B., Yonehara, S., Crump, W. L. & Grimm, E. A. Cell. Immun. 140, 197–205 (1992).
Tsubata, T., Wu, J. & Honjo, T. Nature 364, 645–648 (1993).
Parry, S. L., Hasbold, J., Holman, M. & Klaus, G. G. B. J. Immun. 152, 2821–2829 (1994).
Santos-Argumedo, L., Gordon, J., Heath, A. W. & Howard, M. Cell. Immun. 156, 272–285 (1994).
Holder, M. J. et al. Eur. J. Immun. 23, 2368–2371 (1993).
MacLenna, I. C. M. A. Rev. Immun. 12, 117–139 (1994).
Owen-Schaub, L. B., Radinsky, R., Kruzel, E., Berry, K. & Yonehara, S. Cancer Res. 54, 1580–1586 (1994).
Fisher, D. E. Cell 78, 539–542 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rothstein, T., Wang, J., Panka, D. et al. Protection against Fas-dependent Thl-mediated apoptosis by antigen receptor engagement in B cells. Nature 374, 163–165 (1995). https://doi.org/10.1038/374163a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/374163a0
This article is cited by
-
Mucosal T follicular helper cells in SIV-infected rhesus macaques: contributing role of IL-27
Mucosal Immunology (2019)
-
Second signals rescue B cells from activation-induced mitochondrial dysfunction and death
Nature Immunology (2018)
-
Bcl-2-regulated cell death signalling in the prevention of autoimmunity
Cell Death & Disease (2010)
-
Octamer binding protein 2 (Oct2) regulates PD-L2 gene expression in B-1 cells through lineage-specific activity of a unique, intronic promoter
Genes & Immunity (2010)
-
Signals that influence T follicular helper cell differentiation and function
Seminars in Immunopathology (2010)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.