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KvLQT1 and IsK (minK) proteins associate to form the IKS cardiac potassium current

Nature volume 384, pages 78–80 (1996)Cite this article

Abstract

IN mammalian cardiac cells, a variety of transient or sustained K+ currents contribute to the repolarization of action potentials1. There are two main components of the delayed-rectifier sustained K+ current, IKr (rapid) and IKS (slow)2. IKr is the product of the gene HERG3,4 which is altered in the long-QT syndrome, LQT2 (ref. 5). A channel with properties similar to those of the IKS channel is produced when the cardiac protein IsK is expressed in Xenopus oocytes6–8. However, it is a small protein with a very unusual structure for a cation channel9–15. The LQT1 gene is another gene associated with the LQT syndrome, a disorder that causes sudden death from ventricular arrhythmias16. Here we report the cloning of the full-length mouse KVLQT1 complemen-tary DNA and show that KVLQT1 associates with IsK to form the channel underlying the IKS cardiac current, which is a target of class-Ill anti-arrhythmic drugs and is involved in the LQT1 syndrome.

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References

  1. Deal, K. K., England, S. K. & Tamkun, M. M. Physiol. Rev. 76, 49–67 (1996).

    Article  CAS  Google Scholar 

  2. Sanguinetti, M. C. & Jurkiewicz, N. K. J. Gen. Physiol. 96, 195–215 (1990).

    Article  CAS  Google Scholar 

  3. Sanguinetti, M. C., Jiang, C. G., Curran, M. E. & Keating, M. T. Cell 81, 299–307 (1995).

    Article  CAS  Google Scholar 

  4. Trudeau, M. C., Warmke, J. W., Ganetzky, B. & Robertson, G. A. Science 269, 92–95 (1995).

    Article  ADS  CAS  Google Scholar 

  5. Curran, M. E. et al. Cell 80, 795–803 (1995).

    Article  CAS  Google Scholar 

  6. Takumi, T., Ohkubo, H. & Nakanishi, S. Science 242, 1042–1045 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Folander, K. et al. Proc. Natl Acad. Sci. USA 87, 2975–2979 (1990).

    Article  ADS  CAS  Google Scholar 

  8. Honore, E. et al. EMBO J. 10, 2805–2811 (1991).

    Article  CAS  Google Scholar 

  9. Attali, B. et al. Nature 365, 850–852 (1993).

    Article  ADS  CAS  Google Scholar 

  10. Blumenthal, E. M. & Kaczmarek, L. K. J. Neurosci. 14, 3097–3105 (1994).

    Article  CAS  Google Scholar 

  11. Freeman, L. C. & Kass, R. S. Circ. Res. 73, 968–973 (1993).

    Article  CAS  Google Scholar 

  12. Hice, R. E. et al. Eur. J. Physiol. 426, 139–145 (1994).

    Article  CAS  Google Scholar 

  13. Lesage, F. et al. Recept. Channels 1, 143–152 (1993).

    CAS  PubMed  Google Scholar 

  14. Tzounopoulos, T., Guy, H. R., Durell, S., Adelman, J. P. & Maylie, J. Proc. Natl Acad. Sci. USA 92, 9593–9597 (1995).

    Article  ADS  CAS  Google Scholar 

  15. Zhang, Z. J. et al. Proc. Natl Acad. Sci. USA 91, 1766–1770 (1994).

    Article  ADS  CAS  Google Scholar 

  16. Wang, Q. et al. Nature Genet. 12, 17–23 (1996).

    Article  Google Scholar 

  17. Nuss, H. B. & Marban, E. J. Physiol. (Lond.) 479, 265–279 (1994).

    Article  Google Scholar 

  18. Honore, E., Attali, B., Lesage, F., Barhanin, J. & Lazdunski, M. Biochem. Biophys. Res. Commun. 184, 1135–1141 (1992).

    Article  CAS  Google Scholar 

  19. Benefraim, I., Shai, Y. & Attali, B. J. Biol. Chem. 271, 8768–8771 (1996).

    Article  CAS  Google Scholar 

  20. Attali, B. et al. J. Biol. Chem. 268, 24283–24289 (1993).

    CAS  PubMed  Google Scholar 

  21. Lingueglia, E., Voilley, N., Waldmann, R., Lazdunski, M. & Barbry, P. FEBS Lett. 318, 95–99 (1993).

    Article  CAS  Google Scholar 

  22. Guillemare, E. et al. Biochemistry 31, 12463–12468 (1992).

    Article  CAS  Google Scholar 

  23. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflugers Arch. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  24. Fink, M. et al. J. Biol. Chem. 271, 26341–26348 (1996).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, Valbonne, France

    Jacques Barhanin, Florian Lesage, Eric Guillemare, Michel Fink, Michel Lazdunski & Georges Romey

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  1. Jacques Barhanin
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  2. Florian Lesage
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  4. Michel Fink
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  5. Michel Lazdunski
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  6. Georges Romey
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Barhanin, J., Lesage, F., Guillemare, E. et al. KvLQT1 and IsK (minK) proteins associate to form the IKS cardiac potassium current. Nature 384, 78–80 (1996). https://doi.org/10.1038/384078a0

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