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p85 regulatory subunit of PI3K mediates cAMP–PKA and estrogens biological effects on growth and survival

Oncogene volume 26, pages 2095–2103 (2007)Cite this article

Abstract

Cyclic adenosine 3′5′ monophosphate (cAMP) and protein kinase A (PKA) cooperate with phosphatidylinositol 3′ kinase (PI3K) signals in the control of growth and survival. To determine the molecular mechanism(s) involved, we identified and mutagenized a specific serine (residue 83) in p85αPI3K, which is phosphorylated in vivo and in vitro by PKA. Expression of p85αPI3K mutants (alanine or aspartic substitutions) significantly altered the biological responses of the cells to cAMP. cAMP protection from anoikis was reduced in cells expressing the alanine version p85αPI3K. These cells did not arrest in G1 in the presence of cAMP, whereas cells expressing the aspartic mutant p85D accumulated in G1 even in the absence of cAMP. S phase was still efficiently inhibited by cAMP in cells expressing both mutants. The binding of PI3K to Ras p21 was greatly reduced in cells expressing p85A in the presence or absence of cAMP. Conversely, expression of the aspartic mutant stimulated robustly the binding of PI3K to p21 Ras in the presence of cAMP. Mutation in the Ser 83 inhibited cAMP, but not PDGF stimulation of PI3K. Conversely, the p85D aspartic mutant amplified cAMP stimulation of PI3K activity. Phosphorylation of Ser 83 by cAMP–PKA in p85αPI3K was also necessary for estrogen signaling as expression of p85A or p85D mutants inhibited or amplified, respectively, the binding of estrogen receptor to p85α and AKT phosphorylation induced by estrogens. The data presented indicate that: (1) phosphorylation of Ser 83 in p85αPI3K is critical for cAMP–PKA induced G1 arrest and survival in mouse 3T3 fibroblasts; (2) this site is necessary for amplification of estrogen signals by cAMP–PKA and related receptors. Finally, these data suggest a general mechanism of PI3K regulation by cAMP, operating in various cell types and under different conditions.

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Acknowledgements

This work was partly supported by AIRC (Associazione Italiana Ricerca Cancro), NOGEC (Naples Oncogenomic Center), MIUR (Italian Ministry of Education, University and Research). Concetta Cuozzo is a recipient of an FIRC Fellowship. This work is dedicated to the loving memory of Stelio Varrone.

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Authors and Affiliations

  1. Dipartimento di Biologia e Patologia Molecolare e Cellulare, Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, Università Federico II, Napoli, Italy

    C Cosentino, C Cuozzo, S Agnese, A Feliciello & E V Avvedimento

  2. Dipartimento di Patologia Generale, II Università di Napoli, Napoli, Italy

    M Di Domenico, R Di Stasio & A Migliaccio

  3. Dipartimento di Medicina Sperimentale e Patologia, Università di Roma ‘La Sapienza’, Roma, Italy

    A Porcellini

  4. INM Neuromed, Pozzilli, Italy

    A Porcellini & G De Gregorio

  5. Dipartimento di Neuroscienze-Sezione di Fisiologia, Università Federico II, Napoli, Italy

    M R Santillo

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  1. C Cosentino
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  2. M Di Domenico
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  5. G De Gregorio
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  7. S Agnese
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  9. A Feliciello
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  11. E V Avvedimento
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Correspondence to E V Avvedimento.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Cosentino, C., Di Domenico, M., Porcellini, A. et al. p85 regulatory subunit of PI3K mediates cAMP–PKA and estrogens biological effects on growth and survival. Oncogene 26, 2095–2103 (2007). https://doi.org/10.1038/sj.onc.1210027

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