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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Use of Detergents to Study Membrane Rafts: The Good, the Bad, and the Ugly

  • H. Shogomori and D.A. Brown
From the journal Biological Chemistry

Abstract

Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterol-rich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely used method for studying lipid rafts takes advantage of their insolubility in non-ionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.

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Published Online: 2005-06-01
Published in Print: 2003-09-28

Copyright © 2003 by Walter de Gruyter GmbH & Co. KG

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