Abstract
We show here the identification of Duet, a novel molecule bearing serine/threonine kinase, Dbl-Homology (DH), and Pleckstrin-Homology (PH) domains. The kinase domain of Duet shows a homology to that of DAP kinase that is involved in apoptosis, and Duet is autophosphorylated by an in-vitro kinase assay. The DH- and PH-domains are closely related to those of Trio and Kalirin. Trad mRNA is specifically expressed in skeletal muscle. Duet protein was localized to actin-associated cytoskeletal elements. These data suggest a role of Duet in the cytoskeleton-dependent cell function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Apoptosis Regulatory Proteins
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Calcium-Calmodulin-Dependent Protein Kinases / chemistry
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Carrier Proteins*
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Cloning, Molecular
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Cytoskeleton / enzymology
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Death-Associated Protein Kinases
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Fluorescent Antibody Technique
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GTP-Binding Proteins / chemistry
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Guanine Nucleotide Exchange Factors*
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Humans
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Mice
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Molecular Sequence Data
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Muscle, Skeletal / enzymology
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Phosphoproteins / chemistry
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Phosphorylation
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Protein Serine-Threonine Kinases / chemistry*
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RNA, Messenger / metabolism
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Sequence Analysis, DNA
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Transfection / genetics
Substances
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Apoptosis Regulatory Proteins
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Carrier Proteins
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Guanine Nucleotide Exchange Factors
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Kalrn protein, rat
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Phosphoproteins
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RNA, Messenger
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Trio protein, mouse
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Death-Associated Protein Kinases
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Protein Serine-Threonine Kinases
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TRIO protein, human
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Calcium-Calmodulin-Dependent Protein Kinases
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GTP-Binding Proteins