Elucidating kinetic and thermodynamic constants for interaction of G protein subunits and receptors by surface plasmon resonance spectroscopy

Methods Enzymol. 2002:344:15-42. doi: 10.1016/s0076-6879(02)44703-9.

Authors

R Victor Rebois¹, Peter Schuck, John K Northup

Affiliation

¹ Laboratory of Molecular and Cellular Neurobiology and Biology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.

PMID: 11771379
DOI: 10.1016/s0076-6879(02)44703-9

No abstract available

MeSH terms

Animals
Biotinylation
Brain / metabolism
Cattle
GTP-Binding Protein alpha Subunits, Gi-Go / chemistry
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
GTP-Binding Proteins / chemistry
GTP-Binding Proteins / metabolism*
Kinetics
Protein Subunits
Receptors, Cell Surface / chemistry
Receptors, Cell Surface / metabolism*
Rhodopsin / chemistry
Rhodopsin / metabolism
Surface Plasmon Resonance
Thermodynamics

Substances

Protein Subunits
Receptors, Cell Surface
Rhodopsin
GTP-Binding Proteins
GTP-Binding Protein alpha Subunits, Gi-Go