Abstract
A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108-15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C-terminus. This spliced variant of SSTR2 (designated SSTR2B) encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C-terminus. mRNA corresponding to SSTR2B occurs in mouse tissues in higher abundance than that of SSTR2. SSTR2B binds SS peptides with high affinity when expressed in mammalian cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA / genetics
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Gene Expression
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Glioma
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Humans
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Hybrid Cells
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Kinetics
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Mice
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Molecular Sequence Data
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Neuroblastoma
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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Receptors, Somatostatin / genetics*
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Receptors, Somatostatin / metabolism
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Sequence Homology, Nucleic Acid
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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RNA, Messenger
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Receptors, Somatostatin
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DNA
Associated data
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GENBANK/S39995
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GENBANK/S39996
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GENBANK/S40001
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GENBANK/S40008
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GENBANK/S40009
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GENBANK/S40010
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GENBANK/X58277
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GENBANK/X66432
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GENBANK/X68951
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GENBANK/X70912