Automated fast perfusion of Xenopus oocytes for drug screening

Pflugers Arch. 2006 Oct;453(1):117-23. doi: 10.1007/s00424-006-0125-y. Epub 2006 Sep 5.

Abstract

Fast ('concentration jump') applications of neurotransmitters are crucial for screening studies on ligand-gated ion channels. In this paper, we describe a method for automated fast perfusion of neurotransmitters (or drugs) during two-microelectrode voltage-clamp experiments on Xenopus oocytes. The oocytes are placed in a small bath chamber that is covered by a glass plate with two channels for the microelectrodes that are surrounded by a quartz funnel serving as a reservoir for test solutions. The oocytes are perfused in a vertical direction via the two channels in the plate. Automation of compound delivery is accomplished by means of a programmable pipetting workstation. A mean rise time for 10-90% current increase through muscle-type nACh channels of 55.0+/-1.3 ms (30 muM acetylcholine) was estimated. Automation, fast perfusion rates, and economical use of compounds ( approximately 100 mul/data point) make the system suitable for screening studies on ligand- and voltage-gated ion channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques*
  • Diffusion Chambers, Culture*
  • Drug Evaluation, Preclinical / instrumentation*
  • Drug Evaluation, Preclinical / methods
  • Ligands
  • Oocytes*
  • Perfusion / instrumentation*
  • Perfusion / methods
  • Robotics
  • Time Factors
  • Xenopus

Substances

  • Ligands