[Tyrosinase inhibition of Potentilla bifurca]

Xianglan Piao; Yanze Tian; Xiaoyuan Mi; Jian Cui

[Tyrosinase inhibition of Potentilla bifurca]

Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(15):1952-4.

[Article in Chinese]

Authors

Xianglan Piao¹, Yanze Tian, Xiaoyuan Mi, Jian Cui

Affiliation

¹ Institute of Chinese Minority Traditional Medicine, Central University for Nationalities, Beijing 100081, China. xlpiao@cun.edu.cn

PMID: 19894542

Abstract

Objective: To identify the tyrosinase inhibitory constituent quickly from Potentilla bifurca.

Method: The active constituent was found through fraction collecting and tyrosinase inhibitory activity by bioassay-linked HPLC method.

Result: The methanol extracts and BuOH fraction of Potentilla bifurca showed strong tyrosinase inhibitory activities. From BuOH fraction of Potentilla bifurca, the tyrosinase inhibitory constituent was isolated and identified as flavonoid, quercetin-4'-O-beta-D-glucoside. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.28 mmol x L(-1)) with IC50 value of 0.001 9 mmol x L(-1).

Conclusion: Bioassay-linked HPLC fractionation method was provided for determination the active constituents quickly from herbal medicines.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Inhibitors / chemistry*
Enzyme Inhibitors / isolation & purification
Kinetics
Peptides / chemistry*
Peptides / isolation & purification
Plant Extracts / chemistry
Plant Extracts / isolation & purification
Potentilla / chemistry*

Substances

Enzyme Inhibitors
Peptides
Plant Extracts