Partially deglycosylated equine LH preferentially activates beta-arrestin-dependent signaling at the follicle-stimulating hormone receptor

Mol Endocrinol. 2010 Mar;24(3):561-73. doi: 10.1210/me.2009-0347. Epub 2010 Jan 27.

Abstract

Deglycosylated FSH is known to trigger poor Galphas coupling while efficiently binding its receptor. In the present study, we tested the possibility that a deglycosylated equine LH (eLHdg) might be able to selectively activate beta-arrestin-dependent signaling. We compared native eLH to an eLH derivative [i.e. truncated eLHbeta (Delta121-149) combined with asparagine56-deglycosylated eLHalpha (eLHdg)] previously reported as an antagonist of cAMP accumulation at the FSH receptor (FSH-R). We confirmed that, when used in conjunction with FSH, eLHdg acted as an antagonist for cAMP accumulation in HEK-293 cells stably expressing the FSH-R. Furthermore, when used alone at concentrations up to 1 nM, eLHdg had no detectable agonistic activity on cAMP accumulation, protein kinase A activity or cAMP-responsive element-dependent transcriptional activity. At higher concentrations, however, a weak agonistic action was observed with eLHdg, whereas eLH led to robust responses whatever the concentration. Both eLH and eLHdg triggered receptor internalization and led to beta-arrestin recruitment. Both eLH and eLHdg triggered ERK and ribosomal protein (rp) S6 phosphorylation at 1 nM. The depletion of endogenous beta-arrestins had only a partial effect on eLH-induced ERK and rpS6 phosphorylation. In contrast, ERK and rpS6 phosphorylation was completely abolished at all time points in beta-arrestin-depleted cells. Together, these results show that eLHdg has the ability to preferentially activate beta-arrestin-dependent signaling at the FSH-R. This finding provides a new conceptual and experimental framework to revisit the physiological meaning of gonadotropin structural heterogeneity. Importantly, it also opens a field of possibilities for the development of selective modulators of gonadotropin receptors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arrestins / metabolism*
  • Blotting, Western
  • Cattle
  • Cell Line
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Enzyme Activation / drug effects
  • Female
  • Horses
  • Humans
  • Immunoprecipitation
  • Luteinizing Hormone / analogs & derivatives*
  • Luteinizing Hormone / chemistry
  • Luteinizing Hormone / metabolism
  • Luteinizing Hormone / pharmacology
  • Mice
  • Phosphorylation / drug effects
  • Protein Binding
  • Protein Transport / drug effects
  • RNA, Small Interfering
  • Receptors, FSH / agonists
  • Receptors, FSH / antagonists & inhibitors
  • Receptors, FSH / metabolism*
  • Ribosomal Protein S6 / metabolism
  • Signal Transduction / drug effects*
  • Swine
  • beta-Arrestins

Substances

  • Arrestins
  • RNA, Small Interfering
  • Receptors, FSH
  • Ribosomal Protein S6
  • beta-Arrestins
  • luteinizing hormone, deglycosylated
  • Luteinizing Hormone
  • Cyclic AMP-Dependent Protein Kinases