Abstract
The human promonocytic cell line, U937, when treated for up to 72h with 12,O,tetradecanoyl-phorbol-13-acetate or granulocyte-macrophage colony-stimulating factor, exhibited increased phagocytic activity and expression of the marker p150/95. There was an associated increase in the monocyte proteinase cathepsin B and its mRNA but decreased cellular levels of neutrophil elastase and elastase mRNA. Granulocyte-macrophage colony-stimulating factor therefore causes differentiation of U937 cells, with appropriate effects on the synthesis of leukocyte proteinases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cathepsin B / genetics*
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Cathepsin B / metabolism
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Cell Differentiation / drug effects*
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Cell Line
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Colony-Stimulating Factors / pharmacology*
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Gene Expression Regulation, Enzymologic / drug effects*
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Granulocyte-Macrophage Colony-Stimulating Factor
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Growth Substances / pharmacology*
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Humans
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Kinetics
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Lymphoma, Large B-Cell, Diffuse
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Pancreatic Elastase / genetics*
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Pancreatic Elastase / metabolism
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Phagocytosis / drug effects
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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Tetradecanoylphorbol Acetate / pharmacology*
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Tumor Cells, Cultured / cytology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / enzymology
Substances
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Colony-Stimulating Factors
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Growth Substances
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RNA, Messenger
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Granulocyte-Macrophage Colony-Stimulating Factor
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Pancreatic Elastase
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Cathepsin B
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Tetradecanoylphorbol Acetate