Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

T Kubo; K Fukuda; A Mikami; A Maeda; H Takahashi; M Mishina; T Haga; K Haga; A Ichiyama; K Kangawa

doi:10.1038/323411a0

Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

Nature. 1986 Oct;323(6087):411-6. doi: 10.1038/323411a0.

Authors

T Kubo, K Fukuda, A Mikami, A Maeda, H Takahashi, M Mishina, T Haga, K Haga, A Ichiyama, K Kangawa, et al.

PMID: 3762692
DOI: 10.1038/323411a0

Abstract

Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein. Expression of the complementary DNA produced functional muscarinic receptor in Xenopus oocytes. The muscarinic receptor is homologous with the beta-adrenergic receptor and rhodopsin in both amino-acid sequence and suggested transmembrane topography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Brain / metabolism
Cloning, Molecular
DNA
Nucleic Acid Hybridization
Receptors, Cholinergic / genetics*
Swine
Xenopus

Substances

Receptors, Cholinergic
DNA

Associated data

GENBANK/X04413