Abstract
Intracellular recordings were made from neurones of the rat locus coeruleus in vitro. Acetylcholine increased the firing rate by depolarizing the membrane; both muscarinic and nicotinic antagonists partially reduced this effect. In hexamethonium, pirenzepine shifted the acetylcholine dose-response curve to the right with an equilibrium dissociation constant of 200 nM, indicating an interaction with a m2-type of muscarinic receptor.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylcholine / pharmacology*
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Action Potentials / drug effects
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Animals
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Atropine / pharmacology
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Benzodiazepinones / pharmacology
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Dose-Response Relationship, Drug
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Hexamethonium Compounds / pharmacology
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Locus Coeruleus / cytology*
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Neurons / drug effects*
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Oxotremorine / pharmacology
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Pirenzepine
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Rats
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Receptors, Muscarinic / drug effects*
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Scopolamine / pharmacology
Substances
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Benzodiazepinones
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Hexamethonium Compounds
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Receptors, Muscarinic
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Pirenzepine
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Oxotremorine
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Atropine
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Scopolamine
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Acetylcholine