Abstract
Using a chick P2Y1 receptor cDNA probe we have isolated a mammalian P2Y receptor clone from a bovine aortic endothelial cell library. The sequence has a high degree of similarity to the chick P2Y1 clone. When transfected into the Jurkat cell line, the cDNA conferred sensitivity to purinoceptor agonists. Using fura-2 loaded cells the potency order at the receptor was found to be 2-methylthioadenosine 5' triphosphate = adenosine 5' diphosphate > adenosine 5' triphosphate >> alpha,beta-methyleneadenosine 5' triphosphate and uridine 5' triphosphate. This corresponds to the agonist potency order expected for the bovine aortic endothelial cell P2Y receptor.
MeSH terms
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Adenosine Diphosphate / pharmacology
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / pharmacology
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Amino Acid Sequence
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Animals
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Aorta / chemistry
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Base Sequence
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Calcium / metabolism
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Cattle
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Cell Line
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Chickens
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Cloning, Molecular*
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DNA, Complementary / isolation & purification
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Endothelium, Vascular / chemistry
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Fura-2
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Molecular Sequence Data
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Receptors, Purinergic P2 / chemistry
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Receptors, Purinergic P2 / genetics*
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Receptors, Purinergic P2 / metabolism
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Sequence Homology
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Transfection
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Uridine Triphosphate / pharmacology
Substances
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DNA, Complementary
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Receptors, Purinergic P2
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Adenosine Diphosphate
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Adenosine Triphosphate
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Calcium
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Fura-2
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Uridine Triphosphate