Abstract
Transforming growth factor-beta (TGF-beta) inhibits cell proliferation by inducing a G1-phase cell cycle arrest. Normal progression through G1 is promoted by the activity of the cyclin-dependent protein kinases CDK4 and CDK6 (ref. 2), which are inhibited by the protein p16INK4. We have isolated a new member of the p16INK4 family, p15INK4B. p15 expression is induced approximately 30-fold in human keratinocytes by treatment with TGF-beta, suggesting that p15 may act as an effector of TGF-beta-mediated cell cycle arrest. The gene encoding p15 is located on chromosome 9 adjacent to the p16 gene at a frequent site of chromosomal abnormality in human tumours (9p21).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification*
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Carrier Proteins / metabolism
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Cell Cycle Proteins*
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Cell Cycle*
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Cell Line
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Cloning, Molecular
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase 6
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Cyclin-Dependent Kinase Inhibitor p15
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Cyclin-Dependent Kinase Inhibitor p16
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Cyclin-Dependent Kinases*
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Gene Expression
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Humans
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Keratinocytes / cytology
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Keratinocytes / metabolism
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Molecular Sequence Data
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Protein Serine-Threonine Kinases / antagonists & inhibitors
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Protein Serine-Threonine Kinases / metabolism
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Proto-Oncogene Proteins*
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RNA, Messenger / biosynthesis
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Transforming Growth Factor beta / physiology*
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Tumor Suppressor Proteins*
Substances
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CDKN2B protein, human
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Carrier Proteins
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p15
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Cyclin-Dependent Kinase Inhibitor p16
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Proto-Oncogene Proteins
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RNA, Messenger
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Transforming Growth Factor beta
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Tumor Suppressor Proteins
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Protein Serine-Threonine Kinases
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CDK4 protein, human
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CDK6 protein, human
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase 6
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Cyclin-Dependent Kinases