Abstract
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA, Complementary
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Fluorescence
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Green Fluorescent Proteins
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Luminescent Proteins / chemistry
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Luminescent Proteins / genetics*
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Molecular Sequence Data
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Scyphozoa / genetics*
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Sequence Alignment
Substances
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DNA, Complementary
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Luminescent Proteins
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Recombinant Proteins
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Green Fluorescent Proteins