Abstract
We have cloned cDNA carrying the entire coding sequence of the precursor protein for nociceptin/orphanin FQ, a neuropeptide-ligand for an opioid-receptor like G-protein coupled receptor. The deduced nociceptin/orphanin FQ precursor shows sequence similarity to the opioid peptide precursors and shares characteristic structural features particularly with preprodynorphin. In situ hybridization analysis of nociceptin precursor mRNA in the mouse central nervous system revealed that it is highly expressed in discrete neuronal sites with the pattern distinct from those of opioid peptides.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism*
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Dynorphins / chemistry
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In Situ Hybridization
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Mice
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Molecular Sequence Data
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Neurons / metabolism*
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Nociceptin
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Opioid Peptides / biosynthesis*
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Opioid Peptides / chemistry
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Organ Specificity
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Polymerase Chain Reaction
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Protein Precursors / biosynthesis
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Protein Precursors / chemistry
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Protein Precursors / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Opioid / agonists
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Sequence Homology, Amino Acid
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Spinal Cord / metabolism*
Substances
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DNA Primers
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DNA, Complementary
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Opioid Peptides
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Protein Precursors
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RNA, Messenger
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Receptors, Opioid
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Dynorphins