Abstract
We have cloned and sequenced a 3055-bp human placenta cDNA encoding a P2 purinoceptor. An identical receptor has also been found in human saphenous vein endothelial cells. Comparison of the deduced amino acid (aa) sequence to those of previously described P2Y1 purinoceptors revealed greater than 95% aa identity with the rat and mouse counterparts, and 83% with the chick P2Y1 purinoceptor. Northern blot experiments revealed two mRNAs of 7.5 and 4.6 kb whose expression varies according to the tissue examined.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Conserved Sequence
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DNA, Complementary / genetics
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Endothelium, Vascular / metabolism*
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Female
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Humans
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Mice
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Molecular Sequence Data
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Placenta / ultrastructure
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Pregnancy
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Rats
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Receptors, Purinergic P2 / biosynthesis
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Receptors, Purinergic P2 / genetics*
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Receptors, Purinergic P2Y1
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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DNA, Complementary
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P2RY1 protein, human
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P2ry1 protein, mouse
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P2ry1 protein, rat
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Receptors, Purinergic P2
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Receptors, Purinergic P2Y1