Abstract
The 5' flanking region of a human dihydrodiol dehydrogenase (DDH) gene was isolated and sequenced from bp +124 to -1161. It contains putative binding sites for liver specific factors including NF-IL6 and HNF-5 sites and AP-1, AP-2 and NF kappa B-like sites. Sequence analysis identified this gene as a type II DDH. Reporter analysis of deletion constructs transfected in HepG2 cells identified negative regulatory regions and a minimal promoter (-104 to +65) containing two AP-2 sites in tandem.
MeSH terms
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Base Sequence
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Binding Sites
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CCAAT-Enhancer-Binding Proteins
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Cell Line
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Cloning, Molecular
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Consensus Sequence
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DNA / chemistry
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DNA / metabolism
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DNA-Binding Proteins / metabolism
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Humans
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Liver / metabolism
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Molecular Sequence Data
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NF-kappa B / metabolism
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Nuclear Proteins / metabolism
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Oxidoreductases / genetics*
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Promoter Regions, Genetic*
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Regulatory Sequences, Nucleic Acid
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Sequence Deletion
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Transcription Factor AP-1 / metabolism
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Transcription Factor AP-2
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Transcription Factors / metabolism*
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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NF-kappa B
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Nuclear Proteins
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Transcription Factor AP-1
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Transcription Factor AP-2
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Transcription Factors
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DNA
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Oxidoreductases
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trans-1,2-dihydrobenzene-1,2-diol dehydrogenase