PCR strategy to obtain the 5' and 3' flanking sequences of partial clones from lambda ZAP cDNA libraries

Biotechniques. 1997 Jun;22(6):1038-40, 1042, 1044. doi: 10.2144/97226bm06.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacteriophage lambda / genetics
  • Cloning, Molecular*
  • DNA Primers
  • DNA, Complementary / genetics*
  • Electrophoresis, Agar Gel
  • GTP-Binding Proteins / genetics
  • Gene Library*
  • Plasmids
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Receptors, Purinergic P2 / genetics
  • Turkeys

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Purinergic P2
  • GTP-Binding Proteins