Abstract
Human estrogen receptor beta (hER beta) cDNA that encodes the full-length amino acid sequence has been isolated from testis poly(A)+ RNA with the combination of cDNA screening and reverse transcription-PCR. It is composed of a 1590-bp open reading frame and a segment of the 5'- and 3'-untranslated region (UTR) and encodes an additional 53 amino acids in the N-terminal region compared with the previously reported one. Protein interaction between ER alpha and ER beta was demonstrated in vitro by GST pull-down assay and in vivo by immunoprecipitation. Thus, this study indicates that ER alpha and ER beta can interact in vivo, cross-signaling each other.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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COS Cells
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Cloning, Molecular
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DNA, Complementary / genetics
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Dimerization
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Estrogen Receptor alpha
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Estrogen Receptor beta
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Humans
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In Vitro Techniques
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Male
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Molecular Sequence Data
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Open Reading Frames
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Estrogen / chemistry*
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Receptors, Estrogen / genetics*
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Receptors, Estrogen / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Testis / metabolism
Substances
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DNA, Complementary
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Estrogen Receptor alpha
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Estrogen Receptor beta
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RNA, Messenger
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Receptors, Estrogen
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Recombinant Fusion Proteins