Abstract
The N-hydroxyarylamine O-acetyltransferase from Salmonella typhimurium has been expressed as a histidine-tagged fusion protein in Escherichia coli and purified to apparent homogeneity using single-step immobilized metal ion chromatography. Sufficient quantities of the purified protein have been obtained to allow its characterization by physical methods including dynamic light scattering and electrospray mass spectrometry. The substrate specificity and temperature sensitivity of the enzymatic activity have also been assessed. The enzyme has been crystallized from sodium, potassium tartrate and X-ray diffraction data have been obtained to allow the identification of an orthorhombic unit cell, point group P21212, with dimensions a = 137 A, b = 223 A, and c = 105 A. These crystals will provide a route to a crystallographic determination of the structure of the protein.
Copyright 1998 Academic Press.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases*
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Acyltransferases / chemistry
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Acyltransferases / genetics
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Acyltransferases / isolation & purification*
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Acyltransferases / metabolism
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Aniline Compounds / metabolism
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Base Sequence
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DNA Primers / chemistry
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Gene Expression Regulation, Enzymologic / genetics*
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Isoelectric Point
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Polymerase Chain Reaction
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Salmonella typhimurium / enzymology*
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Salmonella typhimurium / genetics
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Substrate Specificity
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Temperature
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Thrombin / metabolism
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X-Ray Diffraction
Substances
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Aniline Compounds
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DNA Primers
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Recombinant Fusion Proteins
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4-iodoaniline
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Acyltransferases
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Acetyltransferases
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N-hydroxyarylamine O-acetyltransferase
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Thrombin