Mitochondria and cells produce reactive oxygen species in virtual anaerobiosis: relevance to ceramide-induced apoptosis

M Degli Esposti; H McLennan

doi:10.1016/s0014-5793(98)00688-7

Mitochondria and cells produce reactive oxygen species in virtual anaerobiosis: relevance to ceramide-induced apoptosis

FEBS Lett. 1998 Jul 3;430(3):338-42. doi: 10.1016/s0014-5793(98)00688-7.

Authors

M Degli Esposti¹, H McLennan

Affiliation

¹ Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia. mauro.esposti@med.monash.edu.au

PMID: 9688567
DOI: 10.1016/s0014-5793(98)00688-7

Abstract

Observations of apoptosis in virtual anaerobiosis have raised doubts on the significance of reactive oxygen species in the cascade of events of programmed cell death. This work presents evidence that cells and mitochondrial preparations produce similar levels of hydrogen peroxide under either aerobic or virtually anaerobic conditions. These levels are relevant to the increased production of radicals induced by a ceramide analog that promotes apoptosis. This ceramide acts at center o of mitochondrial complex III.

MeSH terms

Anaerobiosis
Animals
Antimycin A / analogs & derivatives
Antimycin A / pharmacology
Apoptosis / physiology*
COS Cells / metabolism*
Cattle
Ceramides / pharmacology*
Electron Transport Complex III / antagonists & inhibitors
Enzyme Inhibitors / pharmacology
Hydrogen Peroxide / metabolism
Mitochondria, Heart / chemistry
Mitochondria, Heart / metabolism
Reactive Oxygen Species / metabolism*
Stilbenes / pharmacology
Submitochondrial Particles / chemistry
Submitochondrial Particles / metabolism*

Substances

Ceramides
Enzyme Inhibitors
Reactive Oxygen Species
Stilbenes
N-caproylsphingosine
3-methoxy-2-(2-styrylphenyl)propenic acid methyl ester
antimycin
Antimycin A
Hydrogen Peroxide
Electron Transport Complex III