Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP

Neuron. 1998 Sep;21(3):581-91. doi: 10.1016/s0896-6273(00)80568-1.

Abstract

We report the cloning of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-binding protein (ABP), a postsynaptic density (PSD) protein related to glutamate receptor-interacting protein (GRIP) with two sets of three PDZ domains, which binds the GluR2/3 AMPA receptor subunits. ABP exhibits widespread CNS expression and is found at the postsynaptic membrane. We show that the protein interactions of the ABP/GRIP family differ from the PSD-95 family, which binds N-methyl-D-aspartate (NMDA) receptors. ABP binds to the GluR2/3 C-terminal VKI-COOH motif via class II hydrophobic PDZ interactions, distinct from the class I PSD-95-NMDA receptor interaction. ABP and GRIP also form homo- and heteromultimers through PDZ-PDZ interactions but do not bind PSD-95. We suggest that the ABP/GRIP and PSD-95 families form distinct scaffolds that anchor, respectively, AMPA and NMDA receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Brain / cytology
  • Brain / growth & development
  • Brain / metabolism*
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Peptide Fragments / chemistry
  • Protein Conformation
  • Rats
  • Receptors, AMPA / chemistry*
  • Receptors, AMPA / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Spinal Cord / cytology
  • Spinal Cord / growth & development
  • Spinal Cord / metabolism*
  • Synapses / metabolism*
  • Synapses / ultrastructure
  • Transcription, Genetic

Substances

  • Carrier Proteins
  • Grip1 protein, rat
  • Grip2 protein, rat
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • Nerve Tissue Proteins
  • Peptide Fragments
  • Receptors, AMPA
  • Recombinant Proteins
  • glutamate receptor ionotropic, AMPA 3
  • glutamate receptor ionotropic, AMPA 2

Associated data

  • GENBANK/AF090113