Abstract
The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg(-1) day(-1)) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / immunology
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
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Animals
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Anion Transport Proteins
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Blotting, Western
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Carrier Proteins / immunology
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Carrier Proteins / metabolism*
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Dexamethasone / administration & dosage
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Dexamethasone / pharmacology*
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Drug Resistance, Multiple
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Gene Expression / drug effects*
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Kidney / drug effects
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Kidney / enzymology
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Kidney / metabolism
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Liver / drug effects
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Liver / enzymology
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Liver / metabolism
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Lung / drug effects
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Lung / enzymology
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Lung / metabolism
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Male
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Membranes / enzymology
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Molecular Weight
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Protein Isoforms / immunology
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Protein Isoforms / metabolism
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Rats
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Rats, Sprague-Dawley
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Anion Transport Proteins
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Carrier Proteins
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Protein Isoforms
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Dexamethasone