RF-amide neuropeptides, with their typical Arg-Phe-NH 2 signature at their carboxyl C-termini,
belong to a lineage of peptides that spans almost the entire life tree. Throughout evolution…

G protein-coupled receptors are thought to mediate agonist-evoked signal transduction by
interconverting between discrete conformational states endowed with different …

Mammalian RF-amide peptides are encoded by five different genes and act through five
different G protein-coupled receptors. RF-amide-related peptides-1 and -3, neuropeptides AF …

Following a recent description of fluorescence resonance energy transfer between enhanced
green fluorescent protein (EGFP)-fused human muscarinic M1 receptors and Bodipy-…

Opioid-induced hyperalgesia and tolerance severely impact the clinical efficacy of opiates
as pain relievers in animals and humans. The molecular mechanisms underlying both …

G protein-coupled receptors (GPCRs) have been described to form hetero-oligomers. The
importance of these complexes in physiology and pathology is considered crucial, and …

Ligand binding to G protein-coupled receptors is a complex process that involves
sequential receptor conformational changes, ligand translocation, and possibly ligand-induced …

Human M1 muscarinic receptor chimeras were designed (i) to allow detection of their
interaction with the fluorescent antagonist pirenzepine labelled with Bodipy [558/568], through …

Tagged biologically active molecules represent powerful pharmacological tools to study and
characterize ligand−receptor interactions. However, the labeling of such molecules is not …

Two fluorescent derivatives of the M1 muscarinic selective agonist AC-42 were synthesized
by coupling the lissamine rhodamine B fluorophore (in ortho and para positions) to AC42-…