Advanced genetic strategies for recombinant protein expression in Escherichia coli

HP Sørensen, KK Mortensen - Journal of biotechnology, 2005 - Elsevier
Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …

[HTML][HTML] Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

HP Sørensen, KK Mortensen - Microbial cell factories, 2005 - Springer
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …

Initiation of protein synthesis in bacteria

BS Laursen, HP Sørensen, KK Mortensen… - Microbiology and …, 2005 - Am Soc Microbiol
Valuable information on translation initiation is available from biochemical data and recently
solved structures. We present a detailed description of current knowledge about the …

[HTML][HTML] Towards universal systems for recombinant gene expression

HP Sørensen - Microbial cell factories, 2010 - Springer
Recombinant gene expression is among the most important techniques used both in
molecular and medical research and in industrial settings. Today, two recombinant expression …

A favorable solubility partner for the recombinant expression of streptavidin

HP Sørensen, HU Sperling-Petersen… - Protein expression and …, 2003 - Elsevier
Recombinant streptavidin is extremely difficult to express at high levels in the cytoplasm of
Escherichia coli without the formation of inclusion bodies. Fusing a solubility enhancing …

Catalytic properties of ADAM12 and its domain deletion mutants

J Jacobsen, R Visse, HP Sørensen, JJ Enghild… - Biochemistry, 2008 - ACS Publications
Human ADAM12 (a disintegrin and metalloproteinase) is a multidomain zinc metalloproteinase
expressed at high levels during development and in human tumors. ADAM12 exists as …

Dialysis strategies for protein refolding: preparative streptavidin production

HP Sørensen, HU Sperling-Petersen… - Protein expression and …, 2003 - Elsevier
We have investigated different dialysis strategies for the refolding of recombinant streptavidin,
and present a novel dialysis setup featuring gradual dilution dialysis and continuous …

Production of recombinant thermostable proteins expressed in Escherichia coli: completion of protein synthesis is the bottleneck

HP Sørensen, HU Sperling-Petersen… - … of Chromatography B, 2003 - Elsevier
Heterologous expression and high yield purification of proteins are frequently required for
structural and functional investigations. Purification of recombinant thermostable proteins is …

Oat (Avena sativa) Seed Extract as an Antifungal Food Preservative Through the Catalytic Activity of a Highly Abundant Class I Chitinase

HP Sørensen, LS Madsen, J Petersen… - Applied biochemistry …, 2010 - Springer
Extracts from different higher plants were screened for the ability to inhibit the growth of
Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena …

[HTML][HTML] Discovery of a novel conformational equilibrium in urokinase-type plasminogen activator

…, E Louise Lange, H Peter Sørensen… - Scientific reports, 2017 - nature.com
Although trypsin-like serine proteases have flexible surface-exposed loops and are known
to adopt higher and lower activity conformations, structural determinants for the different …