Requirement of mammalian DNA polymerase-β in base-excision repair

RW Sobol, JK Horton, R Kühn, H Gu, RK Singhal… - Nature, 1996 - nature.com
SYNTHESIS of DNA by DNA polymerase-β is distributive on single-stranded DNA templates,
but short DNA gaps with a 5′ PO 4 in the gap are filled processively to completion 1,2 . In …

The lyase activity of the DNA repair protein β-polymerase protects from DNA-damage-induced cytotoxicity

…, R Prasad, A Evenski, A Baker, XP Yang, JK Horton… - Nature, 2000 - nature.com
Small DNA lesions such as oxidized or alkylated bases are repaired by the base excision
repair (BER) pathway 1 . BER includes removal of the damaged base by a lesion-specific DNA …

[HTML][HTML] XRCC1 and DNA polymerase β in cellular protection against cytotoxic DNA single-strand breaks

JK Horton, M Watson, DF Stefanick, DT Shaughnessy… - Cell research, 2008 - nature.com
Single-strand breaks (SSBs) can occur in cells either directly, or indirectly following initiation
of base excision repair (BER). SSBs generally have blocked termini lacking the …

Multidrug resistance-associated protein gene overexpression and reduced drug sensitivity of topoisomerase II in a human breast carcinoma MCF7 cell line selected …

E Schneider, JK Horton, CH Yang, M Nakagawa… - Cancer research, 1994 - AACR
A human breast cancer cell line (MCF7/WT) was selected for resistance to etoposide (VP-16)
by stepwise exposure to 2-fold increasing concentrations of this agent. The resulting cell …

[HTML][HTML] Photoaffinity labeling of mouse fibroblast enzymes by a base excision repair intermediate: Evidence for the role of poly (ADP-ribose) polymerase-1 in DNA …

OI Lavrik, R Prasad, RW Sobol, JK Horton… - Journal of Biological …, 2001 - ASBMB
To examine the interaction of mammalian base excision repair (BER) enzymes with DNA
intermediates formed during BER, we used a novel photoaffinity labeling probe and mouse …

[HTML][HTML] Base excision repair intermediates induce p53-independent cytotoxic and genotoxic responses

…, DF Joyce, BP Engelward, JK Horton… - Journal of Biological …, 2003 - ASBMB
DNA alkylation damage is primarily repaired by the base excision repair (BER) machinery in
mammalian cells. In repair of the N-alkylated purine base lesion, for example, alkyl adenine …

[PDF][PDF] HMGB1 is a cofactor in mammalian base excision repair

…, PS Kedar, JK Horton, SI Kanno, K Asagoshi… - Molecular cell, 2007 - cell.com
Deoxyribose phosphate (dRP) removal by DNA polymerase β (Pol β) is a pivotal step in base
excision repair (BER). To identify BER cofactors, especially those with dRP lyase activity, …

Eukaryotic base excision repair: new approaches shine light on mechanism

WA Beard, JK Horton, R Prasad… - Annual review of …, 2019 - annualreviews.org
Genomic DNA is susceptible to endogenous and environmental stresses that modify DNA
structure and its coding potential. Correspondingly, cells have evolved intricate DNA repair …

[HTML][HTML] Protection against methylation-induced cytotoxicity by DNA polymerase β-dependent long patch base excision repair

JK Horton, R Prasad, E Hou, SH Wilson - Journal of Biological Chemistry, 2000 - ASBMB
Using a plasmid-based uracil-containing DNA substrate, we found that the long patch base
excision repair (BER) activity of a wild-type mouse fibroblast extract was partially inhibited by …

[HTML][HTML] Coordination between polymerase β and FEN1 can modulate CAG repeat expansion

Y Liu, R Prasad, WA Beard, EW Hou, JK Horton… - Journal of biological …, 2009 - ASBMB
The oxidized DNA base 8-oxoguanine (8-oxoG) is implicated in neuronal CAG repeat expansion
associated with Huntington disease, yet it is unclear how such a DNA base lesion and …