Effect of dextran on protein stability and conformation attributed to macromolecular crowding

K Sasahara, P McPhie, AP Minton - Journal of molecular biology, 2003 - Elsevier
… Thus, the ΔC P value on the protein folding is affected by the Δc npol and Δc pol that may be
P value for the thermally induced unfolding of hen egg-white lysozyme, the reported ΔC P

Enzymatic aspects of the phenol (aryl) sulfotransferases*

MW Duffel, AD Marshall, P McPhie… - Drug metabolism …, 2001 - Taylor & Francis
… The positions of the PAP/PAPS binding site and the sulfuryl acceptor site as seen from the
structures of PAP and p-nitrophenol (PNP), were modeled by homology to the coordinates for …

The repeat domain of the melanosome fibril protein Pmel17 forms the amyloid core promoting melanin synthesis

…, F Shewmaker, P McPhie… - Proceedings of the …, 2009 - National Acad Sciences
Pmel17 is a melanocyte protein necessary for eumelanin deposition 1 in mammals and
found in melanosomes in a filamentous form. The luminal part of human Pmel17 includes a …

[HTML][HTML] The functional curli amyloid is not based on in-register parallel β-sheet structure

…, RP McGlinchey, KR Thurber, P McPhie… - Journal of biological …, 2009 - ASBMB
The extracellular curli proteins of Enterobacteriaceae form fibrous structures that are
involved in biofilm formation and adhesion to host cells. These curli fibrils are considered a …

[HTML][HTML] Assembly of the yeast cell wall: Crh1p and Crh2p act as transglycosylases in vivo and in vitro

…, O Kosík, N Blanco, J Arroyo, P McPhie - Journal of biological …, 2008 - ASBMB
The cross-linking of polysaccharides to assemble new cell wall in fungi requires mechanisms
by which a preexisting linkage is broken for each new one made, to allow for the absence …

[HTML][HTML] A motif in human histidyl-tRNA synthetase which is shared among several aminoacyl-tRNA synthetases is a coiled-coil that is essential for enzymatic activity …

…, P McPhie, V Sridhar, C Hyde, R Leff, P Plotz - Journal of Biological …, 1994 - Elsevier
In myositis, disease-specific autoantibodies may be directed against an aminoacyl-tRNA
synthetase, usually histidyl-tRNA synthetase. To explore the basis for this phenomenon, we …

An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1, 6-bisphosphate

K Ashizawa, P McPhie, KH Lin, SY Cheng - Biochemistry, 1991 - ACS Publications
… The pellet was suspended in 30 mL of buffer P, which is phosphate-buffered saline free of
… One hundred twenty milliliters of buffer P was added and centrifuged for 30 min at 12000g. …

Domain architecture of the heme-independent yeast cystathionine β-synthase provides insights into mechanisms of catalysis and regulation

KH Jhee, P McPhie, EW Miles - Biochemistry, 2000 - ACS Publications
Cystathionine β-synthase from yeast (Saccharomyces cerevisiae) provides a model system
for understanding some of the effects of disease-causing mutations in the human enzyme. …

Expression of mutant thyroid hormone nuclear receptors is associated with human renal clear cell carcinoma

Y Kamiya, M Puzianowska-Kuznicka, P McPhie… - …, 2002 - academic.oup.com
… For EMSA, identical amounts of TRs were incubated with the 32 P-labeled TRE in the
presence or absence of RXRβ. After electrophoresis, TR homodimers and heterodimers were …

[4] Dialysis

P McPhie - Methods in enzymology, 1971 - Elsevier
Publisher Summary This chapter discusses preparative methods of dialysis in purification of
enzymes. Materials suitable for use as dialysis membranes must be in the form of thin films …