Cytoplasmic DNA triggers activation of the innate immune system. Although 'downstream'
signaling components have been characterized, the DNA-sensing components remain elusive…

Tandem affinity purification (TAP) is a generic two-step affinity purification protocol that
enables the isolation of protein complexes under close-to-physiological conditions for …

Antiviral innate immunity relies on the recognition of microbial structures. One such structure
is viral RNA that carries a triphosphate group on its 5′ terminus (PPP-RNA). By an affinity …

TANK‐binding kinase 1 (TBK1) is of central importance for the induction of type‐I interferon (IFN)
in response to pathogens. We identified the DEAD‐box helicase DDX3X as an …

Dasatinib is a small-molecule kinase inhibitor used for the treatment of imatinib-resistant
chronic myelogenous leukemia (CML). We have analyzed the kinases targeted by dasatinib by …

Genome engineering has been greatly enhanced by the availability of Cas9 endonuclease
that can be targeted to almost any genomic locus using so called guide RNAs (gRNAs). …

Aicardi–Goutières syndrome (AGS) is a rare inherited autoimmune disease caused by
mutations in genes encoding the RNase H2 subunits A, B, and C; the DNase three prime repair …

Knockout collections are invaluable tools for studying model organisms such as yeast.
However, there are no large-scale knockout collections of human cells. Using gene-trap …

Near-haploid human cell lines are instrumental for genetic screens and genome engineering
as gene inactivation is greatly facilitated by the absence of a second gene copy. However, …

Viruses must enter host cells to replicate, assemble and propagate. Because of the restricted
size of their genomes, viruses have had to evolve efficient ways of exploiting host cell …