… centrifuged (15,000g for 3 min), and 50 μl of the supernatant was transferred into a new
sterile tube containing 30 μl of 20× buffer (3M NaCl/0.3 M citrate, pH 7.4) and 20 μl of 37% (w/w) …

… and Knepel, W., Release of fllipotropin- and fl-endorphin-like material induced by angiotensin
in … 15 Knepel, W. and Meyer, DK, Effect of isoprenaline and trimethidinJum on the plasma …

The glucagon gene is expressed specifically in the alpha cells of the pancreatic islets. We
show here that 300 base pairs of the 5′-flanking region of the rat glucagon gene, linked to a …

… RNA was fixed to the membrane by W cross-linking. Blots were prehybridized … A cknow ~
e ~ ~ en ~ s - W e greatly appreciate the generous gift of unique reagents From the following …

Background It has been suggested that stress provokes neuropathological changes and may
thus contribute to the precipitation of affective disorders such as depression. Likewise, the …

The 5′-flanking region of the rat glucagon gene contains, from nucleotides–291 to–298, a
sequence (TGA CGTCA) which mediates cyclic AMP (cAMP) responsiveness in several …

Clinical observations agree that antidepressant drugs are ef-fective only after a lag phase of
1-3 weeks. This delay could be explained at the molecular level by an action on gene …

Gene transcription can be induced by cAMP and Ca 2+ through distinct protein kinases
phosphorylating the transcription factor CREB, which binds to cAMP response elements (CREs) …

Glucagon-producing pancreatic islet cells generate calcium-dependent action potentials. By
the control of calcium influx through voltage-gated calcium channels, calcium is a tightly …

Although kappa-opiate receptors represent an important fraction of the total opiate receptor
capacity in human brain their endocrine function is unknown. We determined the effects of a …