CRISPR/Cas9 Genome Editing of the Human Topoisomerase IIα Intron-19 5′ Splice Site Circumvents Etoposide Resistance in Human Leukemia K562 Cells

Data Supplement

• Supplemental Data -

  Supplemental Table 1 - Sequences of primers, single strand oligonucleotides, and qPCR hybridization probes utilized in this study.

  Supplemental Table 2 - Cell line growth characteristics and etoposide-induced growth inhibition.

  Supplemental Figure 1 - TOP2α/170 and TOP2α/90 protein levels in parental K562 cells, drug resistant K/VP.5 cells, and gene-edited clonal cell lines K/VP.5/edit-1, K/VP.5/edit-2, and K/VP.5/edit-3.

  Supplemental Figure 2 - Growth inhibitory effects of etoposide in K562, K/VP.5 and gene-edited clonal cell lines.

  Supplemental Figure 3 - Scatter plot of gene expression between gene-edited K/VP.5/edit-3 and K/VP.5 cells for 625 essential genes (denoted in blue) (Wang et al., 2019a) and the top 20 putative Cas9/g-RNA-2 off-target genes predicted by the CCTop algorithm (https://cctop.cos.uni-heidelberg.de/) (Stemmer et al., 2015) and expressed in K/VP.5/edit-3 and K/VP.5 cells. Dotted lines denote 2 fold-change in gene expression.