Data Supplement
- Supplemental Figures -
Supplementary Figure 1 - Sequence alignment of the CaS and mGluR5 intracellular domains.
Supplementary Figure 2 - Western blot analysis of CaS in HEK-293 cells stably transfected with either wild-type or mutant CaS cDNAs.
Supplementary Figure 3 - Effect of CaSS875A mutation on Ca2+i mobilisation following stable transfection into HEK 293 cells.
Supplementary Figure 4 - CaST888A exhibits enhanced Ca2+i mobilisation.
Supplementary Figure 5 - Relative membrane localisation of receptor mutants CaSS875A and CaST888A following transient transfection in HEK-293 cells.
Supplementary Figure 6 - Effect of CaSS875A/T888A double mutation on Ca2+i mobilisation following transient transfection into HEK-293 cells.
Supplemental Figure 7 - CaSS875A/T888A double mutation does not enhance muscarinic receptor responsiveness.
Supplemental Figure 8 - Ca2+i mobilisation response for the ECD truncated mutant, Rho-C-hCaS.