Data Supplement
- Supplemental Data -
Supplementary Figure 1: Mass spectrometry of coregulator RID proteins purified and used in completive anisotropy.
Supplementary Figure 2: Proportion of genes that are significantly differentially expressed.
Supplementary Figure 3: Principle component analysis of all differentially expressed genes and transcripts.
Supplementary Figure 4: Non-ligand bias mechanisms do not account for observed biased signaling.
Supplementary Figure 5: PPARγ can be blocked with the covalent inverse agonist T0070907.
Supplementary Figure 6: Analysis of T0070907-blocked genes.
Supplementary Figure 7: Rosiglitazone and GW1929 do not consistently induce changes from DMSO in HEK293T cells at 3 hours.
Supplementary Figure 8: Competitive anisotropy reaches equilibrium at 2 hours at room temperature.
Supplementary Figure 9: Power analysis indicates that 8 replicates will provide a power of 0.8 at an FDR of 0.05.
Supplementary Table 1: Previous reports of putative PPARγ biased agonists in animal models.
Supplementary Table 2: Q-TOF mass spectrometry masses of proteins expressed and purified from E. coli.
Supplementary Table 3: Characteristics of RNA-seq Experiments.
Supplementary Table 4: Selectivity index genes converted to human gene names.
Supplementary Table 5: Selectivity Index.
Supplementary Table 6: PPARγ ligands – doses used experimentally.
Supplementary Methods 1: Derivation of occupancy equation and assumptions used in the derivation.
- Supplemental Data File 1 -
Data and calculations relevant to Figure 1 panel d and e.
- Supplemental Data File 2_3h -
RNAseq data for 3-hour ligand treatment.
- Supplemental Data File 2_24h -
RNAseq data for 24-hour ligand treatment.
- Supplemental Data File 3 -
KEGG pathway analysis, including biased signaling.
- Supplemental Data File 4 -
Data relevant to Supp. Figures 4, 5, and 7.