Abstract
The effects of clofibrate on the growth, glycogen content, triglyceride content, and levels of some peroxisomal enzymes of the ciliated protozoan Tetrahymena pyriformis have been studied. Clofibrate sensitivity inhibited growth and depleted the cell glycogen. The triglyceride content of Tetrahymena in Ringer's solution in the absence of an exogenous carbon source was doubled by exposure to clofibrate for 2 hr. Cells grown in the presence of clofibrate for 17 hr showed a 30% increase in isocitric dehydrogenase activity, a 30% decrease in isocitric lyase activity, and a 20% increase in catalase activity. The results are consistent with the hypothesis that clofibrate inhibits gluconeogenesis or activates glycogenolysis.
ACKNOWLEDGMENTS This work was supported by grants from the National Science Foundation (No. GB-2788) and the National Institutes of Health (No. 5-RO1-HD-01269). Dr. Blum is the recipient of a Research Career Development Award (No. K3-GM-2341). Mr. Wexler is a predoctral trainee, supported by a Public Health Service training grant (No. 5-T01-GM-00929) from the National Institutes of Health. Miss Delores Randolph and Mr. Alvernon Hayes provided excellent technical assistance. We are also grateful to Dr. B. Wittels for instruction in the techniques of lipid analysis.
- Copyright ©, 1968, by Academic Press Inc.
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