Abstract
Chick embryonic liver cells grown in monolayer cultures metabolize aminopyrine to 4-aminoantipyrine, which is released into the medium. This N-demethylation is performed by liver cell cultures but not by renal or intestinal cell cultures from chick embryos. The formation of 4-aminoantipyrine is linear with time and is inhibited by SKF 525-A (2-diethylaminoethyl 2,2-diphenylvalerate HCl), piperonyl butoxide, hexobarbital, lauric acid, and testosterone. Inhibition in all cases is at least partially reversible. The rate of 4-aminoantipyrine formation is also reversibly inhibited by carbon monoxide. From the CO:O2 ratio and rate of formation of the metabolite, it is possible to estimate the partition coefficient, KL, for cytochrome P-450 in living cells, which agrees quite well with estimates from incubations in vitro.
ACKNOWLEDGMENT We would like to thank Mrs. Sandra Weinstein for excellent technical assistance.
- Copyright ©, 1971, by Academic Press, Inc.
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