Abstract
By extending previous studies of the phenobarbital (PB)-responsive 132-base pair (bp) enhancer sequence in the CYP2B10gene, we have delimited a 51-bp enhancer element that is fully inducible by PB in mouse primary hepatocytes. Sixteen structurally unrelated phenobarbital-type inducers activated the 51-bp enhancer element in transient transfection assays. The results thus indicate that most PB-type inducers, if not all inducers, increase the transcription of the CYP2B10 gene by activating this 51-bp element, now designated PB-responsive enhancer module or PBREM.
Footnotes
- Received December 12, 1997.
- Accepted January 9, 1998.
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Send reprint requests to: Dr. Masahiko Negishi, Pharmacogenetics Section, Lab of Reproductive and Developmental Toxicology, NIEHS, NIH, Research Triangle Park, NC 27709. E-mail:negishi{at}niehs.nih.gov
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↵1 Current affiliation: Department of Pharmaceutics, University of Kuopio, FIN-70211 Kuopio, Finland.
- The American Society for Pharmacology and Experimental Therapeutics
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